Fig. 4: Visualization of chromosome targeting of td5oxStayGold-tagged condensin I at low copy number expressed via a genome-editing technique.
From: StayGold variants for molecular fusion and membrane-targeting applications

After release from cell cycle arrest, genome-edited HCT116 cells (#897) were imaged for CAP-H-td5oxStayGold (at 488 nm excitation) and SiR-DNA-labeled chromosomes (at 637 nm excitation) using spinning-disk LSCM (SpinSR10) at the indicated times (min:s). Every 1 min, three-dimensional (3D) scanning was executed with a z step size of 1 μm over an axial range of 13 μm and the green and far-red fluorescence images were merged. Maximum intensity projection (MIP) images are shown. Time-series image data of the cell (bottom) boxed in the entire field of view (top). Representative of n = 3 independent experiments. Scale bars, 10 μm. See Supplementary Video 2.