Extended Data Fig. 4: gGRABDA3h sensors report optogenetically-elicited DA release in the mouse mPFC.
From: Improved green and red GRAB sensors for monitoring dopaminergic activity in vivo
a, Schematic illustration depicting the experimental design for panel b-i. b, Histological verification of indicated sensor expression in mPFC and ChrimsonR expression in VTA. Dashed boxes indicate the ___location of optical tract. Scale bar, 1 mm. c, Representative fluorescence changes and zoom-in view (indicated by dashed box) of indicated sensors during optogenetic stimulations under control condition or in the presence of SCH-23390 (SCH). d, Average traces of the change in gDA3h (top) or dLight1.3b (bottom) fluorescence from a mouse. Data are shown as mean±s.d. e, Group summary of ΔF/F0 for the indicated sensors. n = 4 mice for gDA3h and dLight1.3b, respectively, mean±s.e.m. One-way ANOVA, post hoc Tukey’s test was performed. **p = 0.0035 for gDA3h; n.s. p = 0.9122 for dLight1.3b; *p = 0.0295 between gDA3h and dLight1.3b. f, Group summary of the rise and decay time constant of the gDA3h signals in response to optogenetic stimulations. n = 4 mice, mean±s.e.m. g, h, Example fluorescence response (g) and corresponding average traces (e) of gDA3h (top) or dLight1.3b (bottom) to indicated optogenetic stimulation. The average traces are shown as mean±s.d. i, Group summary of peak ΔF/F0 of gDA3h or dLight1.3b in response to indicated optogenetic stimulation. n = 4 mice for gDA3h and dLight1.3b, mean±s.e.m.
