Extended Data Fig. 7: Streptavidin hexapods do not stimulate T cells in the presence and absence of shear stresses.
From: Multimodal probing of T-cell recognition with hexapod heterostructures
Intracellular staining and ELISA were used to measure IFN-γ (a), TNF-α (b), and IL-2 (c) expressions. Additionally, surface staining measured CD25 (d) and CD69 (e) expressions in OT-1 T cells after a 12-hour incubation under various experimental conditions, as indicated by colors. Histograms show the expression of primary OT-1 CD8+ T cells, with sextuplicate independent experiments performed. Bar graphs were generated to depict the percentage of positive cells (left), mean fluorescence intensity (MFI) (middle), and cytokine level (right). These graphs were based on sextuplicate data obtained from staining primary CD8+ T cells. All statistical analyses were performed using one-way ANOVA tests paired with Bonferroni’s multiple comparisons. Data are represented as the mean value ± SEM with individual replicates (n = 6 independent repeats).
