Extended Data Fig. 3: Time-resolved WAXS data collected with low noise levels recorded at the SPB/SFX instrument of the European XFEL.

Our data shows an exceptional signal-to-noise ratio, allowing for difference signals of ~10−6 to be recorded. As the normalization conditions alter the scale of the difference signal, the data is typically normalized around 1.5 Å−1 or 2.3 Å−1. Here, the data is normalized to the scattering in the range between 1.6 Å−1 and 1.4 Å−1 to match the data treatment of previously published time-resolved solution scattering studies performed at a synchrotron, where best-case noise levels are ~10−4 and ~5*10−5 in pump-probe and sequential acquisition schemes. The data shown here,ΔI(q), have a q-bin size of 0.00836 Å−1. The red lines at the pre-excitation time point show ± 10−5, respectively; this boundary corresponds to the noise levels in the data.