Extended Data Fig. 5: Segmentation examples of EpH4 mouse epithelial cells lamellae medium magnification montages. | Nature Methods

Extended Data Fig. 5: Segmentation examples of EpH4 mouse epithelial cells lamellae medium magnification montages.

From: Smart parallel automated cryo-electron tomography

Extended Data Fig. 5

Shown are 3 examples of medium magnification montages of lamellae milled from EpH4 mouse epithelial cells (left) and their segmentations using the SPACEtomo model trained on S. cerevisiae (right). While some classes (for example ice contamination, dark and bright areas, coating) are successfully segmented by the Yeast model, cellular classes are not reliably detected, and even large areas of cytoplasm are classified as background.

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