Supplementary Figure 2: Supplementary data relating to cell-type-specific LH→NAc functional connectivity mapping
From: Accumbal D2 cells orchestrate innate risk-avoidance according to orexin signals
A, B. Probing orexin cell→D1 cell interaction. A,Targeting strategy (top) and resulting expression of mCherry (red) in LH (bottom), representative example of n = 5 brains showing expression localization typical of orexin cells. B, Recording scheme (top), and effects of low and high frequency orexin axon photo-stimulation on D1 cells (representative example of n = 48 neurons, 48/48 cells showed no detectable responses). C, D. Probing MCH→D2 cell interaction. C, Targeting strategy (top) and resulting expression of mCherry (red) in LH (bottom), representative example of n = 5 brains showing expression localization typical of MCH cells. D, Recording scheme (top), and effects of low and high frequency MCH axon photo-stimulation on D2 cells (representative example of n = 24 neurons, 24/24 cells showed no detectable responses).
