Supplementary Figure 3: Probing sufficiency of NAc orexin for innate risk-avoidance behaviors
From: Accumbal D2 cells orchestrate innate risk-avoidance according to orexin signals
A. Experimental paradigm: orexin or control vehicle solutions were infused bilaterally into accumbens via implanted cannulae, followed by behavioral assays, see Supplementary Methods. B. Effect of orexin on center preference in the open-field test. Left and center, examples of spatial exploration heatmaps from the same mouse infused with orexin or vehicle solutions. Right, effect of orexin or vehicle infusion on centre occupancy (% time during the first 15 min of arena exploration) in n = 10 mice. P value is from a two-tailed paired t test, t = 3.545 df = 9. In the same tests, orexin did not significantly affect locomotion velocity (vehicle = 7.24+/-3.5 cm/sec; orexin = 6.04+/-0.96 cm/s; p = 0.176 by two-tailed paired t-test, t=1.468 df=9). C. Effect of orexin on hesitation in the PORT test. Left and center, examples of spatial exploration heatmaps from the same mouse infused with orexin or vehicle solutions. Right, effect of orexin or vehicle infusion on hesitation to entry of middle (odor) chamber in n = 10 mice. P value is from a two-tailed paired t test, t = 4.281 df = 9.
