Supplementary Figure 4: Long-term stability of orientation and temporal frequency tuning in RVΔGL-Cre-labeled neurons.
From: Nontoxic, double-deletion-mutant rabies viral vectors for retrograde targeting of projection neurons
Visual responses of cells taken from Fig. 5a (lower FOV) are plotted for three different time points. Each panel (a–d) shows the orientation tuning of one cell at 7 (top), 54 (middle), and 122 days (bottom row) postinjection, obtained with drifting gratings presented at 8 directions of motion and 5 temporal frequencies (TF) (same stimuli as in Fig. 5; mean ∆F/F ± s.e.m., averaged over 15 repeats). The FOVs of the three imaging sessions (e–g) show the cells circled and labeled with their respective panel letter. Both the orientation tuning curves and temporal frequency preferences remain strikingly similar over four months of imaging. This was consistent across our data set of 9 FOVs. Axis labels in (a) apply to all plots. Scale bar: 100 μm.
