Supplementary Figure 5: Synaptic properties of cINs transplanted into mouse cerebral cortex.

Related to Fig. 2. The lines used in these experiments are summarized in Supplementary Table 3. (a) Left: Grafted human interneurons were recorded in acute brain slices. Grafted cells, identified with green fluorescence (GFP⁺), receive excitatory synaptic inputs from host neurons. Right: Representative trace of postsynaptic responses recorded in a GFP⁺ grafted cell. Spontaneous excitatory postsynaptic currents (sEPSCs) were recorded in GFP⁺ grafted cells at –85 mV in voltage-clamp mode in the healthy control (black traces) and schizophrenia groups (red traces). The experiment was repeated independently in 26 cells with similar results. (b) The frequency of sEPSCs in grafted cINs from the healthy control (12 cells) and schizophrenia groups (14 cells). (c) The amplitude of sEPSCs in grafted cINs from the healthy control (12 cells) and schizophrenia groups (14 cells). (d) Left: blue light illumination evokes action-potential firings and induces GABA release in GFP⁺ interneurons expressing ChR2, generating inhibitory postsynaptic responses in GFP^– host neuron (Rec). Right: the amplitude of inhibitory postsynaptic currents induced by photostimulation of grafted cINs from the healthy control (15 cells) and schizophrenia groups (13 cells). In (b)-(d), graphs in left panels indicate data combined for each group (healthy control and schizophrenia; mean ± SEM), whereas plots in right panels indicate data split for each line (mean ± standard deviation). Circles indicate data obtained from each individual cell. For detailed statistics information, see Supplementary Table 15.