Extended Data Fig. 2: ZIKV infects NPCs in vitro.
From: AHR is a Zika virus host factor and a candidate target for antiviral therapy
a, NPCs were infected with ZIKV (MOI = 1) and 48 h postinfection, cells were fixed for immunofluorescence against ZIKV NS5 (green). Nuclei were counterstained with DAPI (blue). Images were acquired using an Olympus FV1000 confocal laser scanning microscope. Scale bar = 50 µm. Images are representative of 3 independent experiments. b, AHR modulation in NPCs. NPCs were treated with I3S or CH223191. 24 h after treatment, NPCs were harvested for qPCR analysis of AHR, CYP1A1, IDO1 and TDO2. Data represent the mean ± SD (n = 3 independent experiments). P values were determined by a two-sided Student’s t-test.
