Extended Data Fig. 10: hTDP-43 interacts with TREM2 in vivo in mouse brain.

GFP-hTDP-43 was expressed in the primary motor cortex of 2-month-old mice via stereotactic intracerebral injection of AAV9.CAG.hTDP-43.GFP. a, Representative images of co-localization of TREM2 (red) with Iba1 (white) in microglia phagocytosing GFP-hTDP-43 (green) in the primary motor cortex of indicated groups at 14 dpi. Arrowheads indicate co-localization of TREM2 with phagocytic puncta of GFP-hTDP-43. Scale bar, 20 µm. b, SPR analysis of the binding between recombinant hTREM2 ECD (Met1-Ser174) with NFG and AMM at the indicated concentrations.