Extended Data Fig. 6: Generation of Adra1afl/fl mice.
From: Norepinephrine links astrocytic activity to regulation of cortical state
Top: schematic of the genetic strategy used to generate conditional deletion of Adra1a. Primers (green arrows) were designed to identify and distinguish between the endogenous and knock-in Adra1a alleles. Bottom: Numbers are in base pairs. Validation of the knock-in strategy used to generate Adra1afl/fl mice. PCR of Adra1afl/+ mice generates both a 154 base pair band corresponding to the wild type allele and a 235 base pair band corresponding to successful LoxP knock-in, which is not found in wild-type mice. N = 5 Adra1afl/+ and 4 wild type mice.
