Extended Data Fig. 5: Relationship between dendrites and long-range axonal projection for PT and CT neurons.
(a) Correlation between total lengths of dendrites and axons for IT, PT, and CT neurons (linear regression, P value and R2 are shown). Shaded areas around the regression line indicate 95% confidence intervals. P values were calculated via two-sided t-test. (b) Correlations between the pair-wise distances of dendrite morphology based on multi-dimensional morphological features and the pair-wise distances of axon projections calculated by FNT-dist (Methods) for IT, PT, and CT neurons, respectively (linear regression, P value and R2 are shown). P values were calculated via two-sided t-test. (c) Pearson’s correlation between dendrite morphological features and axon projection bias (MY vs ACB) of PT neurons in AI/ORBl/ORBvl. (d) Representative MY-projecting or ACB-projecting neurons of different dendrite/axon subtype. (e) Soma distributions of the neurons in different PT dendrite subtypes and the gradient of their dendrite morphology/axon projection pattern. Left, soma distributions of PT neurons on the PFC flatmap with dendrite subtypes color-coded. The shaded area denotes the AI/ORBl/ORBvl. Middle and right, correlations of apical trunk length between soma position (in lateral-medial axis of AI/ORBl/ORBvl) or axon projection pattern (MY-projecting or ACB-projecting) for the PT dendrite subtypes (linear regression, P value and R2 are shown). Shaded areas around the regression line indicate 95% confidence intervals. P values were calculated via two-sided t-test. (f) The distributions of axon projection strength to major targets of CT dendrite subtypes with their soma distributions in PFC subregions. Top, hierarchical tree of CT dendrite subtypes based on their soma distribution (Euclidean distance, Ward’s linkage). Middle, dot plot shows the number of neurons (represented by dot size) in PFC subregions. Bottom, violin plot shows the axon projection strength. Colored by dendrite subtypes. (g) Pearson’s correlation between dendrite morphological features and axon projection bias to ventral medial nucleus of thalamus (VM) vs. reticular nucleus of thalamus (RT) of CT neurons in ACA/PL/MOs. (h) Representative VM-projecting or RT-projecting neurons of different dendrite/axon subtype. (i) Soma distributions of the neurons in different CT dendrite subtypes and the gradient of their dendrite morphology/axon projection pattern. Left, soma distributions of CT neurons on the PFC flatmap with dendrite subtypes color-coded. The shaded area denotes the ACA/PL/MOs. Middle and right, correlations of the height of dendrite between soma position (in anterior-posterior axis of ACA/PL/MOs) or axon projection pattern (VM-projecting or RT-projecting) for the CT dendrite subtypes (linear regression, P value and R2 are shown). Shaded areas around the regression line indicate 95% confidence intervals. P values were calculated via two-sided t-test.
