Extended Data Fig. 5: Microglial subpopulations were not significantly activated by dissociation enzyme and considered as ‘artifactual’. | Nature Neuroscience

Extended Data Fig. 5: Microglial subpopulations were not significantly activated by dissociation enzyme and considered as ‘artifactual’.

From: Integrating spatial and single-nucleus transcriptomic data elucidates microglial-specific responses in female cynomolgus macaques with depressive-like behaviors

Extended Data Fig. 5

a, the number (left) or proportion (right) of microglia were not significantly different between these three groups. b, boxplot showed a significantly increased PIMID proportion in DLs (Controls, n = 7; DLs, n = 7; RES, n = 5. box=25-75th percentiles, whiskers=Tukey, horizontal line in box=median). c, activation score based on the exAM signature gene modules and plotted on UMAP coordinates. d, expression of exAM gene markers in microglia subpopulations. 10 genes (SOCS3, CCL3, CCL4, KLF2, HIST2H2AA1, HIST1H4I, HSPA1A, HSPA1B, HIST1H2BC, HIST1H1C) were not expressed in any microglia. e, scoring results of microglia subpopulations based on the activation score in each subpopulation.

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