Extended Data Fig. 6: PIMID expression signatures.

a, normalized expression of 4 marker genes of PIMID. b, overview of dlPFC slices. Each slice contained clear cortical structures, including edges, grey matter and white matter. n = 5 per group. c, Immunofluorescence staining and quantification of IQGAP2⁺ microglia in dlPFC. IQGAP2⁺IBA1⁺ colocalization cells were identified as Mic03 cells. IQGAP2 was identified as a gene marker of Mic03 (FDR = 1.095 e-212) in sn-RNA data. IBA1 was a widely used microglial marker. The IQGAP2⁺ microglia were identified following a criterion of IQGAP2⁺IBA1⁺ cells with clear microglial features including long processes and a central soma. Two representative cells were photographed at 40X magnification, IQGAP2⁺IBA1⁺(left, yellow arrow) and IBA1⁺ microglia (right, white arrow).