Extended Data Fig. 9: Unsupervised clustering of dlPFC ST hybridization spots showed layer distribution.

a, HE staining of dlPFC ST slices (Controls, n = 3; DLs, n = 2; RESs, n = 3). The boxes indicated the hybridized spot area (6.5 mm×6.5 mm). b-i, spot clusters showed layer distribution. Left, the merged plots of HE-stained image and in situ clusters. Right, the UMAP plots of each cluster.