Fig. 4: Time-resolved activity-regulated gene expression in cued fear conditioning.
From: Neuronal types in the mouse amygdala and their transcriptional response to fear conditioning
a, Tone-cued fear conditioning and scRNA-seq sampling timepoints after 2 h, 8 h, 24 h and 2 h after next-day conditioned stimulus (CS) recall (no shock). HC, naïve HC control. b, Per timepoint, fraction and cell number (left) of cells highly expressing IEGs Arc, Bdnf, Btg2, Fos, Fosl2, Homer1, Npas4 or Nr4a1 (IEGhigh, 99th percentile); observed versus the fraction and number expected by chance, shown for the five top-responding and all clusters. Right, resultant ratio (observed/expected), per timepoint. c, Time-dynamic expression (fold change) of the eight IEGs, 90th percentile, for the five top IEG-scoring clusters among GABA (top) and GLUT (bottom) types. d, Cluster-wise IEG score (Methods), visualized as heatmap on t-SNE (as in Fig. 1d). Blue, low; red, high and gray, cluster too small (<60 cells). Gray label/dashed circle, smallest group size <20 cells. Circle size represents cluster size.
