Extended Data Fig. 9: PKM2 deletion results in the switch from aerobic glycolysis to oxidative phosphorylation.
a-f, Oligomycin A and 2-DG were applied to compare OXPHOS and glycolytic contributions to the ATP level after PKM2 deletion. The ex vivo brain slices were treated with 10 μM Oligomycin A at 1.5 min and 3.5 mM 2-DG at 9 min until the end of imaging at 18 min. Fluorescence image of the SSp brain slices expressed with iATPSnFR to record the ATP level at 0 min, 9 min, and 18 min (a). Time-lapse interactive confidence lines of the iATPSnFR fluorescence in the SSp with a 99% confidence interval (b). The somatic OXPHOS index (OXPHOS/(OXPHOS + glycolysis)) with (n = 26 somata from 3 mice) or without (n = 23 somata from 3 mice) injection of AAV-hSyn-Cre in the SSp, p < 0.0001 (c). Fluorescence image of the SNc brain slices expressed with iATPSnFR to record the ATP level at 0 min, 9 min, and 18 min (d). Time-lapse interactive confidence lines of the iATPSnFR fluorescence in the SNc with a 99% confidence interval (e). The somatic OXPHOS index (OXPHOS/(OXPHOS + glycolysis)) with (n = 22 somata from 3 mice) or without (n = 20 somata from 3 mice) injection of AAV-TH-Cre in the SNc, p < 0.0001 (f). For the boxplots, the centre line indicates the median, the box limits indicate the first and third quartiles, and the whiskers indicate the data range. For interactive confidence interval line plots, the centre for the error bands indicates the mean. Scale bars, 50 μm (a and d) and 3 min (b and e). ***p < 0.001. Student’s two-tailed unpaired t-test (c and f).
