Extended Data Fig. 6: Female Mef2c L35P+/- mice exhibited hyperactive locomotion without anxiety behavior.
From: Whole-brain in vivo base editing reverses behavioral changes in Mef2c-mutant mice
(a) Representative locomotion track traces of female Mef2c WT and L35P+/- mice in the open field test. Quantitative analysis of total distance (b), mean velocity (c), and duration in the center zone (d) of Mef2c WT and L35P + /- mice in the open field test (n = 8, b: P = 0.0054; c: P = 0.0054). (e) Representative locomotion track traces of female Mef2c WT and L35P+/- mice in the light/dark exploration test. (f) Quantitative analysis of cumulative duration of female Mef2c WT and L35P+/- mice in the light area (n = 8 per group). Representative heat maps of Mef2c WT and L35P+/- mice in the elevated plus maze (open arms are top and bottom arms; closed arms are left and right). (g) Quantification of cumulative duration exploring in the open arm (h) and closed arm (i) of female Mef2c WT and L35P+/- mice (n = 8, h: P = 0.0114; i: P = 0.038). (j) Representative locomotion track traces of female Mef2c WT and L35P+/- mice in the Barnes maze. (k) Quantitative analysis of primary latency spent locating the target hole of female Mef2c WT and L35P+/- mice in the Barnes maze (n = 8 per group). Quantification of cumulative duration exploring in the target (l) and opposite zone (m) of female Mef2c WT and L35P+/- mice in the apparatus (n = 8 per group). n represents mice number. Statistical values represent the mean ± s.e.m. * P < 0.05, ** P < 0.01, (b, c, d, f, h, i, l, m) unpaired two-tailed Student’s t-test, (k) Two-way ANOVA test.
