Extended Data Fig. 7: Design of AeCBE.
From: Whole-brain in vivo base editing reverses behavioral changes in Mef2c-mutant mice
(a) Illustration of various designs for classic CBE and APOBEC3A-embedding CBE with different embedding positions. (b) Screening for base editing efficiency of various APOBEC3A-embedding CBEs using sgRNA indicated in the HEK293 cells. (c) Representative FACS gating strategy for GFP, mCherry, and double positive samples in Fig. 3c. Quantitative analysis of base editing efficiency (C-to-T conversion) of bystander sites C103(d), C108(e), and C112(f) after different CBE applications in cultured mouse cortical neurons (n = 3 per group; n represents independent experiments, One-way ANOVA test). Statistical values represent the mean ± s.e.m.
