Extended Data Fig. 3: Decreased protein expression and aberrant inhibitory interneuron development in Mef2c L35P+/- mice.
From: Whole-brain in vivo base editing reverses behavioral changes in Mef2c-mutant mice
(a) Design of Mef2c L35P knock-in mice by CRISPR/Cas9 mediated homologous recombination. (b) Representative images of immunohistochemical staining for PV (red), Mef2c (green) and DAPI (blue) in the retrosplenial cortex (white dotted box indicates corresponding brain region) of Mef2c WT and L35P+/- mice. Scale bars, 200μm. Quantitative analysis of PV-positive neuronal cell density in the retrosplenial cortex (c), dentate gyrus (d), the somatosensory cortex (e), and visual cortex (f) of Mef2c WT (n = 11 brain slices from 4 mice) and L35P+/- mice (n = 12 brain slices from 4 mice). (c: P < 0.0001; d: P < 0.0001; e: P = 0.0004; f: P < 0.0001) (g) Representative images of immunohistochemical staining for SST (red), Mef2c (green), and DAPI (blue) in the retrosplenial cortex (White dotted box indicates corresponding brain region) of Mef2c WT and L35P+/- mice. Scale bars, 200μm. Quantification of SST-positive neuronal cell density in the retrosplenial cortex (h), hippocampus (i), and somatosensory cortex (j) of Mef2c WT (n = 8 brain slices from 3 mice) and L35P+/- mice (n = 7 brain slices from 3 mice). Statistical values represent the mean ± s.e.m. ***P < 0.001, **** P < 0.0001, (c, d, e, f, h, i, j) unpaired two-tailed Student’s t-test.
