Fig. 2: dPR1 and TN1A-2 are activated during pulse song induced by stimulation of the descending neurons pIP10.

a, Expression pattern of the split-LexA driver for pIP10. Similar expression patterns were observed in three flies. Scale bars, 50 μm. b, Example of the sound induced by optogenetic activation of pIP10 in the single fly optogenetic activation experiment. The shaded area represents the period of LED stimulation. c, Example ΔF/F trace of a dPR1 neuron (top) together with the simultaneously recorded sound (bottom). jGCaMP7s was expressed with dsx-Gal4 and calcium signals were recorded from a dPR1 cell body. d, Time course of dPR1 ΔF/F and song around transitions from quiet to pulse song production. Dashed vertical lines represent the transition time. Data are from 12 neurons in 6 flies and represented as mean ± s.e.m. across transitions for both ΔF/F and song (n = 190 transitions). e, The mean change in ΔF/F after quiet-to-pulse transitions (see Methods for details) for dPR1. Each dot represents a neuron. Lines represent mean ± s.d. across neurons (n = 12 neurons in 6 flies). f–h, Same as c–e but for TN1A-2. GCaMP was expressed with the TN1A-2 split-Gal4 line. g, n = 99 transitions from 13 neurons in 4 flies. h, n = 13 neurons in 4 flies. See also Extended Data Fig. 4.