Fig. 5: Song-type selective signals in the motor circuit are carried by a genetically defined population of neurons.
From: Activity of nested neural circuits drives different courtship songs in Drosophila
a, Schematic of the imaged region. For each fly, images were obtained from either dorsal or medial regions of the VNC. CsChrimson and GCaMP were expressed using the song driver and fru-Gal4, respectively. b, The mean ΔF/F during pulse (left) and sine (middle) song around song-type transitions for the dorsal part of the VNC in an example fly (n = 202 transitions). Images were averaged across z-planes and time. Scale bar, 50 μm. c, Population-averaged ΔF/F for the difference between pulse and sine song around song-type transitions for the recordings from the dorsal part of the VNC (n = 6 flies). Scale bar, 50 μm. d, Histogram of the mean difference in ΔF/F between pulse and sine song during song-type transitions for the voxels that changed ΔF/F depending on song type (P < 0.001, two-sided permutation test). Data of the recordings from the dorsal part of the VNC were combined (n = 6 flies). A histogram expected by chance was obtained using a randomization method (‘Trial shuffle’; Methods). e,f, Same as c (e) and d (f) but for the recordings from the medial part of the VNC (n = 5 flies). See also Extended Data Fig. 7.
