Extended Data Fig. 1: TYK2 regulates tau concentrations in cells.
From: TYK2 regulates tau levels, phosphorylation and aggregation in a tauopathy mouse model
a–c, Immunoblotting (IB) image and quantitative graph showing reduction of tau levels upon either TYK2 knockdown using shRNA (n = 6, one-way ANOVA, Dunnett’s multiple comparison test, P(NT-TYK2 shRNA1) = 0.0087, P(NT-TYK2 shRNA2) = 0.0045; a) or TYK2 catalytic inhibition using a small molecule (deucravacitinib, BMS986165; n = 6, one-way ANOVA, Dunnett’s multiple comparison test, P(DMSO-TYK2 inhibitor, 50 nM) = 0.0058, P(DMSO-TYK2 inhibitor, 100 nM) = 0.0007; b). In contrast, TYK2 overexpression increases endogenous tau levels in a neuroblastoma cell line (n = 8, unpaired t test, two-tailed, P = 0.0001; c). n is the number of biological replicates. Data are represented as mean ± SEM (**p < 0.01, ***p < 0.0005).
