Extended Data Fig. 7: Axon collaterals of dACC→PnC neurons and behavioral tests with photoactivation or photoinhibition of the dACC→PnC pathway.
From: A top-down slow breathing circuit that alleviates negative affect in mice
a, Axon collaterals of dACC→PnC neurons observed in regions other than the PnC. Scale bar, 200 μm. b,c, Photoactivation of dACC→PnC neurons (ChR2, N = 6; eGFP, N = 7 male mice) did not change approach response to female odor (b) but reduced avoidance response to TMT (c). d–f, Photoactivation of dACC→PnC neurons during light/dark choice test (d, ChR2, N = 9 and eGFP control, N = 8 mice). Photoactivation of dACC→PnC neurons reduces ΔBPM (light–dark; e) and increases time spent in the light zone (f). g,h, Photoinhibition of dACC→PnC projections (eNpHR3.0, N = 7; eGFP control, N = 8 male mice) did not change approach response to female odor (g) or avoidance response to TMT (h). LV, lateral ventricle; CPu, caudate putamen; cc, corpus callosum; D3V, dorsal third ventricle; PVT, paraventricular thalamic nucleus; MD, mediodorsal thalamic nucleus; VMT, ventromedial thalamic nucleus; DpME, deep mesencephalic nucleus; RMC, red nucleus, magnocellular; ZI, zona incerta; LH, lateral hypothalamus; mt, mammillothalamic tract; ic, internal capsule. N.SP > 0.05, *P < 0.05, **P < 0.01. Bar graphs are shown as mean ± s.e.m. Box-whisker plots are shown as median and interquartile range with minimum and maximum.
