Extended Data Fig. 9: Measurement of breathing rate during photoactivation of PnCGABA terminals.
From: A top-down slow breathing circuit that alleviates negative affect in mice
a, Inductance plethysmography experiments performed with photoactivation of PnCGABA→VLM terminals (ChR2, N = 7; eGFP, N = 7 mice), tested with different frequencies. b, Changes in breathing rates (% of baseline) induced by stimulation of PnCGABA→VLM terminals (ChR2, N = 7; eGFP, N = 7 mice). c, Inductance plethysmography experiments performed with photoactivation of PnCGABA→LHb/PVT terminals (ChR2, N = 7; eGFP, N = 8 mice), tested with different frequencies. d, Changes in breathing rates (% of baseline) induced by stimulation of PnCGABA→LHb/PVT terminals (ChR2, N = 7; eGFP, N = 8 mice). e–g, Breathing rates shown as breaths per minute (BPM) during photoactivation experiments (15-Hz stimulation; e, PnCGABA→VLM; f, PnCGABA→LHb/PVT; g, AAV1-based labeling). ***P < 0.001. Line graphs are shown as mean ± s.e.m. Box-whisker plots are shown as median and interquartile range with minimum and maximum.
