Fig. 2: Neurogenic programs are preserved in the aging DG.
a, UMAP visualization of the whole neurogenic lineages. b, Relative proportions of each neurogenic cell type in the mouse DG. c, Expression of cell-type-specific genes. Color gradient indicates the log-normalized gene expression level. d, Heatmap shows gene expression dynamics of neurogenic lineage progression across pseudotime. Numbers in circle indicate gene modules. Color gradient indicates the z-score of gene expression. e, SNN graphs of the whole neurogenic populations (left) and the individual age (right) showing that the uni-directional neurogenic trajectory is preserved in advancing ages as well as the temporal expression patterns of individual pseudotime modules. f, Violin plots showing three classes of DEGs between astrocytes and qNSCs. Upper, shared genes include Slc1a3, Sox2 and Gfap; middle, astrocyte-specific genes include S100b, Kcnk1 and Igfbp2; lower, qNSC-specific genes include Ascl1, Stmn1 and Cd9. g, Immunofluorescent staining showing that most radial NSCs (GFAP+SOX2+) labeled by Nestin-GFP in the SGZ across all three ages do not overlap with astrocytic marker S100b (n = 3 mice for each condition). Arrowheads indicate the soma of qNSCs. Scale bars, 20 μm. MO, months old.
