Extended Data Fig. 3: Module activity scoring is comparable with existing gene co-expression metrics. | Nature Neuroscience

Extended Data Fig. 3: Module activity scoring is comparable with existing gene co-expression metrics.

From: Integrated analysis of molecular atlases unveils modules driving developmental cell subtype specification in the human cortex

Extended Data Fig. 3

a and b) Selected modules with cell type-specific activity patterns as measured by our module activity score demonstrated comparable activity patterns when evaluated using AUCell or Module Score. Module expression patterns evaluated by ModuleEigenegene differed from these three metrics, likely due to its unique focus on other metrics besides gene expression. a) UMAPs showing scores for module activity, AUCell, Module Score, and Module Eigengene across the developmental meta-atlas. b) Boxplots comparing the scores of these four metrics across developmental stages in deep layer and other neuronal subtypes. With the exception of Module Eigengene, deep layer neurons display an early, sustained increase in activity across all scoring algorithms.

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