Extended Data Fig. 6: AQuA2 detects small and large myelin Ca²⁺ transients that are mGluR5-regulated.

A: Relative frequency distribution of myelin Ca²⁺ transients of different sizes (1 µm² bins), after 1 h CHPG and DMSO treatment (DMSO n = 85; CHPG n = 95). B: Frequency of myelin Ca²⁺ transients after 1 h of CHPG treatment separated by size (less than or greater than 1 µm²) (DMSO N = 17; CHPG N = 17) (One-way Anova p-value = 0.0084; Dunn’s multiple comparisons test; DMSO < 1 µm² vs. < 1 µm² CHPG p-value = >0.9999; DMSO < 1 µm² vs. CHPG < 1 µm² p-value = 0.5854; DMSO < 1 µm² vs. > 1 µm² CHPG p-value = 0.3726; DMSO > 1 µm² vs. > 1 µm² CHPG p-value = 0.0384; DMSO < 1 µm² vs. > 1 µm² CHPG p-value = 0.0200; CHPG < 1 µm² vs. > 1 µm² CHPG p-value = >0.9999). DMSO < 1 µm² mean: 28.3, SD: ±25.71; DMSO > 1 µm² mean: 18.69 SD: ±25.11; CHPG < 1 µm² mean: 66.06, SD: ±73.09; CHPG > 1 µm² mean: 71.96, SD: ±78.67). C: Avg. Ca²⁺ transients amplitude comparison of small ( < 1 µm²) Ca²⁺ transients after 1 h treatment with DMSO (N = 9, mean: 0.82, SD: ±0.21) and CHPG (N = 12, mean: 1.08, SD: ±0.25) (Mann–Whitney test; p-value = 0.0148). D: Comparison of large ( > 1 µm²) Ca²⁺ transients amplitude after 1 h treatment with DMSO (mean: 0.87, SD: ±0.16) and CHPG (mean: 0.99, SD: ±0.18) (Unpaired t-test; p-value = 0.0853).

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