Extended Data Fig. 2: GMM validation.
From: Psilocybin-enhanced fear extinction linked to bidirectional modulation of cortical ensembles
a, Smoothed distributions (Gaussian, window of 4 for nonimplanted and 3 for implanted mice) of percentage freezing during the trace period in late Extinction 3. Dashed red line represents the inferred threshold from a GMM of both saline and psilocybin datasets. b, Cartoons depicting hypothesized sub-distributions composing saline and psilocybin distributions. c, Probability of assignment to low-freezing groups of mice across 10,000 iterations of GMMs using the leave-one-out method for each animal in each iteration. d, Effect of model choice on key result. Percent freezing during the trace period in early Extinction 3 was compared using thresholds from GMMs trained on only saline mice, psilocybin mice, both or the average threshold of each treatment’s GMM. (F(treatment)(1,10) = 9.774, P = 0.0108; mixed effects RM model with Sidak correction; Supplementary Table 2 (rows 1). e. Accuracy of animal classification across session periods. Dashed line is chance (50%).
