Supplementary Figure 3: Conservation analysis and mutagenesis of the MukB elbow. | Nature Structural & Molecular Biology

Supplementary Figure 3: Conservation analysis and mutagenesis of the MukB elbow.

From: A folded conformation of MukBEF and cohesin

Supplementary Figure 3

a, Sequence alignment of the N-terminal (left) and C-terminal (right) parts of the MukB elbow. Residues chosen for mutagenesis are highlighted by triangles. Eco, Escherichia coli; Mmo, Morganella morganii; Tmo, Thioflavicoccus mobilis; Emo, Endozoicomonas montiporae; Tau, Tolumonas auensis; Osp, Oceanimonas sp. GK1; Btr, Bibersteinia trehalosi; Hdu, Haemophilus ducreyi. b, Sequence conservation (Jensen-Shannon divergence) was mapped onto the structure (high conservation is purple, low conservation is cyan). c, Growth of strains containing point mutations at the elbow in the endogenous mukB gene. d, Construction of a functional mukB-HaloTag allele. e, Protein levels of elbow mutants fused to a HaloTag. Extracts were labelled with a HaloTag-TMR substrate and were analyzed by in-gel fluorescence (top) and Coomassie staining (bottom) after SDS-PAGE. WT, wild-type.

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