Supplementary Figure 5: AcrVA5 inhibits the Cas12a strain with lysine but not arginine for recognition of PAM.
From: An anti-CRISPR protein disables type V Cas12a by acetylation
a, Time course of cleavage inhibition assays were used to test the inhibition activities of AcrVA5 against WT and R608K mutant Lb2Cas12a. The reactions were stopped by adding loading buffer for denaturing gel and the reaction mixtures were run on TBE-urea polyacrylamide gels and visualized by ethidium bromide staining. Lb2Cas12a from strain Lachnospiraceae bacterium COE1 denotes a close LbCas12a ortholog (45% identity). b, Time course of cleavage inhibition assays were used to test the inhibition activities of AcrVA5 against WT and K595R mutant LbCas12a. The reactions were stopped by adding loading buffer for denaturing gel and the reaction mixtures were run on TBE-urea polyacrylamide gels and visualized by ethidium bromide staining.
