Extended Data Fig. 10: Effects of amantadine binding on ETM.

The peptide is reconstituted in DMPC: DMPG membranes with an AMT: ETM monomer molar ratio of 8: 1. a, 2D ¹⁵N-¹³Cα correlation spectra of apo (blue) and AMT-bound ETM (magenta). The spectra were measured at 305 K under 14 kHz MAS. Zoomed-in areas show peaks with significant CSPs. b, 2D ¹³C-¹³C correlation spectra with 20 ms mixing of apo (blue) and AMT-bound ETM (magenta). The spectra were measured at 263 K. Zoomed-in areas shows peaks with significant CSPs. The perturbed residues are concentrated in the N- and C-termini of the protein. c, 1D ¹⁹F direct-polarization spectra of 3F-AMT with and without the peptide in DMPX membranes. The spectra were measured at 270 K under 14 kHz MAS. d, ¹³Cα selective ¹⁹F-dephased REDOR spectra of AMT-bound ETM in DMPC: DMPG membranes. The ΔS spectra show dephasing at 65.5 ppm, 63.6 ppm, 56 ppm and 54 ppm. e, Broadband ¹³C-¹⁹F REDOR spectra. The ΔS spectra show ¹³C dephasing for sidechains that belong to residues that show Cα dephasing in (d).