Extended Data Fig. 10: Model for TSS scanning.
From: Structures of transcription preinitiation complex engaged with the +1 nucleosome
a. The PIC-nucleosome complex structure presented here corresponds to the state before scanning starts (pre-scanning state). The Pol II active center and the TSS are indicated. The lower panel shows a schematic representation. Distances between the TATA box and selected DNA elements are denoted. b. Model of the PIC-nucleosome complex in the post-scanning state. The modelled structure was generated using structures of the yeast PIC with open DNA (PDB code: 7O75)9 and of Pol II with a partially unraveled nucleosome (PDB code: 6A5T)23. The partially unraveled nucleosome with 5 turns of nucleosomal DNA detached from the histone octamer position was aligned with the PIC-nucleosome structure based on DNA and manually adjusted to avoid clashes between the nucleosome and TFIIH.
