Extended Data Fig. 3: In vitro assembly kinetics of immature particles using different IPs.
From: HIV-1 is dependent on its immature lattice to recruit IP6 for mature capsid assembly
(A–C) In vitro assembly of 75 µM ΔMA-CANC using IPs at indicated concentrations at 25 °C. (D) Negative stain EM images of immature particles from A-C. Scale bar = 200 nm. (E–G) In vitro assembly of immature VLPs at indicated ΔMA-CANC and IP concentrations sufficient to maintain 1:1 stoichiometry (1 IP per 1 hexamer) at 25 °C. (H) In vitro assembly with purified ΔMA-CANC at 75 µM hexamer and 12.5 µM (1:1 stoichiometry) IP3 or IP6 at 37 °C. At the indicated time point, an additional 12.5 µM of IP3 or IP6 was added. When additional IP6 but not IP3 is added to the reaction there is a renewed increase in light scattering indicative of further assembly. (I) As with (H), but at the indicated time point excess IP3 is added (500 µM) leading to a resumption in assembly to IP6-stimulated levels.
