Fig. 2: The SGO1–CES interaction protects centromeric sister chromatid cohesion. | Nature Structural & Molecular Biology

Fig. 2: The SGO1–CES interaction protects centromeric sister chromatid cohesion.

From: Structural basis of centromeric cohesion protection

Fig. 2

a, Zoomed-in view of the CES binding pocket of SA2 (blue) and SCC1 (green) bound to SGO1 (yellow). b, Computational model containing SA1 (gray). c, Representative images of different chromosome phenotypes during prometaphase. d, Quantification of chromosome phenotypes in prometaphase wild-type cells and SGO1Y335A F337A cells (unpaired t test; ****P < 0.0001). e, Quantification of the chromosome phenotypes in prometaphase wild-type, SA1W337A, SA2W334A and SA1W337A SA2W334A cells (unpaired t test; ***P ≤ 0.0004, ****P < 0.0001, NS, not significant). f, Quantification of chromosome phenotypes in prometaphase wild-type and SGO1Y335A F337A cells after treatment with either siLuciferase (siLuc), siSGO1 or siWAPL. All panels depict the mean ± s.d. of three independent experiments with more than 70 cells analyzed per experiment (unpaired t test; ****P < 0.0001).

Back to article page