Fig. 3: The SGO1–CES interaction dictates SGO1 localization.
a, Representative immunofluorescence images of GFP-tagged wild-type SGO1 or SGO1Y335A F337A (green) and CENPA (magenta) upon treatment with nocodazole. Scale bar, 5 μm. b, Quantification of centromeres with GFP signal enriched between CENPA signal of the two chromatids (dark gray column) or GFP signal enriched at the CENPA signal (light gray column) in cells transfected with GFP-tagged wild-type SGO1 or SGO1Y335A F337A. We analyzed four random centromeres over 30 cells. This experiment was performed three times; mean ± s.d. c, Quantification of the mean ± s.d. of the intensity of SGO1-GFP (yellow) and CENPA (magenta) along the centromeric region on cells treated with nocodazole. The intensity at each point was normalized to the highest intensity measured per chromosome. The point between two CENPA signals was established as the reference point for each measurement. We analyzed four random centromeres over 30 cells. This experiment was performed three times. d, Schematic representation of the predominant phenotype observed in (a–c). S, SGO1. e, Representative images of SGO1–GFP wild type (green) ___location with respect to CENPA (magenta) in WAPL-depleted HAP1 wild-type and SA1W337A SA2W334A cells upon nocodazole treatment. Scale bar, 5 μm. f, Quantification of the images depicted in e, using analysis methods as in b. We analyzed four random centromeres over 30 cells. This experiment was performed three times; mean ± s.d. g, Quantification of the images depicted in e, using analysis methods as in c. h, Schematic representation of the predominant phenotype observed in e–g.
