Extended Data Fig. 8: PDHE1α recruited to chromatin facilitates DNA damage repair and maintains genome integrity.
From: PARylated PDHE1α generates acetyl-CoA for local chromatin acetylation and DNA damage repair
a, Comet assay showing the DSB repair efficiency in WT and PDHE1α-KO HeLa cells treated with etoposide (10 μM). Representative images are shown in the left panel. Relative tail moments of ≥100 cells were quantified using ImageJ. The center lines of the box-and-whisker plots show the medians, box limits indicate the 25th and 75th percentiles and whiskers extend 1.5x the interquartile range from the 25th and 75th percentiles. The median values of each group are listed under the boxplots. Scale bars, 50 μm. b, Representative gating strategy used to analyze DNA repair efficiency (left) and flow cytometry data for the DR-U2OS and EJ5-U2OS reporter assays shown in Fig. 6B. c, IF staining showing the kinetics of γH2AX foci formation and disappearance from WT and PDHE1α-deficient HeLa cells treated with etoposide (10 μM). The Representative images of γH2AX foci are shown (left), and the number of foci (≥100 cells) was quantified (right) from three independent experiments. Scale bars, 5 μm.
