Extended Data Fig. 3: Protein–lipid overlay analysis.
From: Structure and activation of the RING E3 ubiquitin ligase TRIM72 on the membrane
a-d, Protein–lipid overlay assay using PIP StripsTM (a), Inositol Snoopers® (b), Oxidized Phospholipid Snoopers® (c) and Sphingolipid Snoopers® (d) in the absence (left) or presence (middle) of Ca2+. The spotted lipid positions are indicated in the right panel. e, Lists of the examined lipids. TRIM72 WT was incubated and detected with a rabbit polyclonal anti-TRIM72 antibody. The detailed experimental procedures are described in the Methods.
