Extended Data Fig. 2: DNA methylation dynamics during diapause and reactivation.
From: TET activity safeguards pluripotency throughout embryonic dormancy
a. Global clustering of WGBS samples. b. DNA methylation levels of different genomic features in wild-type ESCs over time during mTORi treatment. White dots show the median; vertical box plots within violin plots show interquartile range (IQR) and the whiskers show 1.5 IQR. c. Immunofluorescence of untreated or mTORi treated ESCs for 5mC and 5hmC methylation. Bottom, single-nucleus quantifications of 5mC and 5hmC intensities normalized to DAPI. n, number of cells. Statistical test is two-tailed t-test. d. Immunofluorescence of E4.5, in vivo diapaused, and reactivated (48 h post in vivo diapause) mouse blastocysts for 5mC methylation. Right, single-nucleus quantifications of 5mC intensity normalized to DAPI. Horizontal line shows the median, box spans the IQR and whiskers span 1.5 IQR. n, number of cells. Statistical test is a one-way Anova with Tukey’s multiple comparison test. Dashed lines mark the ICM.
