Extended Data Fig. 4: Conservation of FHIP1B and putative binding to Rab5.
From: KIF1C activates and extends dynein movement through the FHF cargo adapter
a, ConSurf86 evolutionary conservation profile of FHIP1B. Model of FHIP1B coloured according to level of conservation (key below) and the view of the structure is the same as that in (b). b, AlphaFold2 prediction of FH571-718F+Rab5Q79L. Predicted structure was superimposed on experimental cryo-EM FHF structure and used to show the interaction with Rab5 in three different views (top row middle and right panels, and bottom row, middle panel). Boxed inset shows predicted ___domain boundaries of Rab5 based on87. c, AlphaFold2 Predicted Aligned Error (PAE) plot of FH571-718F+Rab5Q79L.
