Fig. 6: Motility behavior of dynein and kinesin-driven cocomplexes.
From: KIF1C activates and extends dynein movement through the FHF cargo adapter
a, Cartoons depicting the proteins used in each plus end-directed cocomplex. b–d, Superplots showing plus end-directed speed (b), run lengths (c) and dwell time (d) of KIF1C–HOOK3 complexes (HKFL) in the presence of dynein and dynactin (DDHKFL), dynein tail and dynactin (DTDHKFL) and dynactin only (xDHKFL). n = 94, 95, 113 and 137, respectively. The small dots show data per microtubule analyzed (n) and the large dots show experimental averages. The boxes show quartiles, whiskers show 10–90% of data and the median is highlighted by the orange line. The exact P values shown above graphs using Kruskal–Wallis H test followed by a Conover’s post hoc test to evaluate pairwise interactions with a multiple comparison correction applied using Holm–Bonferroni. e, Cartoons depicting the proteins used in each minus end-directed cocomplex. f–h, Superplots showing minus end-directed speed (f), run lengths (g) and dwell time (h) of DDH complexes in the presence of either KIF1C full-length (DDHKFL) or KIF1C stalk (GST–KIF1C stalk–GFP, DDHKS). n = 165, 197 and 199, respectively. The small dots show data per microtubule analyzed (n) and large dots show experimental averages. The boxes show the quartiles, the whiskers show 10–90% of data and the median is highlighted by the orange line. The exact P values shown above the graphs were calculated using the Kruskal–Wallis H test followed by a Conover’s post hoc test to evaluate pairwise interactions with a multiple comparison correction applied using Holm–Bonferroni.
