Extended Data Fig. 1: Controls for single molecule experiments.
From: KIF1C activates and extends dynein movement through the FHF cargo adapter
Representative kymographs from single molecule motility assays of Dynein-Dynactin-HOOK3-KIF1C (DDHK) complexes showing a, the full DDHK complex, and omission of individual components with b, TMR-dynein omitted (xDHK) and c, dynactin (DxHK) and d, HOOK3-Alexa 647 (DDxK) and e, KIF1C-GFP (DDHx). The omitted factor is shown in grey in the cartoons and crossed out in the component list. Microtubule polarity is indicated with (+) and (–) signs. Note that motility of KIF1C and Dynein both towards the plus and minus end of microtubules is only observed in the complete DDHK complex.
