Extended Data Fig. 2: Native PAGE screening for suitable substrates for PolθH-DNA cryo-EM studies.
From: Human polymerase θ helicase positions DNA microhomologies for double-strand break repair
a, An example gel from Native PAGE screening for DNA substrates that bind PolθH, consecutively stained with Diamond DNA stain (top) and Coomassie protein stain (bottom). This experiment was independently repeated three times with similar results. Gel migration of each species is labeled on the left, and the key is on the right. MH = microhomology. b, The stem-loop DNA species pursued for structural studies, with 6 bases of self-complementary microhomology (green) at the end of the 3′ overhang.
