Extended Data Fig. 2: ChIP-seq profiles of UAD-2 and USTC in C. elegans; piRNA gene localization correlates with expression in C. briggsae.
From: piRNA gene density and SUMOylation organize piRNA transcriptional condensate formation
a. Heatmap of ChIP-seq binding profiles of UAD-2 around type I and type II piRNA transcription start sites (TSSs). b. Heatmap of ChIP-seq binding profiles of TOFU-4 around type I and type II piRNA transcription start sites (TSSs). c. Heatmap of ChIP-seq binding profiles of TOFU-5 around type I and type II piRNA transcription start sites (TSSs). d, A flowchart for UMI piRNA sequencing. e, Genome-wide distribution of piRNA genes (n = 25883) in C. briggsae. f, Boxplots presenting log2(mean of piRNA reads from two biological replicates) for Cbr-PRG-1-associated piRNAs across Cluster 1, Cluster 2, Cluster 3, and out-Cluster in C. briggsae. The box itself represented the interquartile range between 5% and 95%, thereby encompassing the middle 90% of the data set. The central horizontal line and the adjacent numeric value within the box represent the mean value. The star within the box represents the median value. Error bars represent the mean ± 1.5 standard deviations (SD), indicating the variability of the data around the mean. Outliers are individually marked as points, representing data points that fall outside the range of mean±1.5 SD. Statistical significance was determined using a two-sample t test. g, Graphs presenting the fractional distribution of piRNA genes and their corresponding expression reads (normalized as reads per million from two biological replicates) across Cluster 1, Cluster 2, Cluster 3, and out-Cluster in C. briggsae. Source data are provided as a Source Data file.
