Extended Data Fig. 3: CRISPR/Cas9-mediated chromosome inversion and translocation alter piRNA expression.

a, Diagram showing sequence details of intrachromosomal inversion in LG IV. b, Graphs presenting the expression levels of total piRNAs from Cluster I of the indicated animals, based on average reads from two biological replicates, normalized as reads per million+1. c-e, Scatter plots displaying the expression levels of Cluster I, Cluster II, and out-Cluster piRNAs in the wild-type (x-axis) and inversion strain (y-axis), based on the log₂(average piRNA reads of two biological replicates), normalized as reads per million+1. f, Boxplot revealing log₂(average piRNA reads of two biological replicates) for both the wild-type and inversion strains across Cluster I, Cluster II, and out-Cluster loci. Normalized as reads per million+1. The box itself represented the interquartile range between 5% and 95%, thereby encompassing the middle 90% of the data set. The central horizontal line and the adjacent numeric value within the box represent the mean value. The star within the box represents the median value. Error bars represent the mean ± 1.5 standard deviations (SD), indicating the variability of the data around the mean. Outliers are individually marked as points, representing data points that fall outside the range of mean±1.5 SD. Statistical significance was determined using a paired-sample t test.