Extended Data Fig. 7: Forward genetic screening identifies factors suppressing UAD-2 foci formation.

a, Forward genetic screening procedures for UAD-2::GFP regulators at 20 °C and 25 °C. b, Complementation test for two alleles of gei-17 at 25 °C. All images were taken by the Leica THUNDER imaging System and deconvoluted using Leica Application Suite X software (version 3.7.4.23463). All images are representative of more than three animals (see also in Extended Data Fig. 7g and h). c, Expression levels of total piRNAs from LG IV in WT worms fed L4440 and smo-1 dsRNA at 25 °C, normalized as reads per million+1. d, Boxplots illustrating log₂(piRNA reads per million+1) for WT worms fed with L4440 and smo-1 dsRNA at 25 °C from a single biological replicate. The box itself represented the interquartile range between 5% and 95%, thereby encompassing the middle 90% of the data set. The central horizontal line and the adjacent numeric value within the box represent the mean value. Error bars represent the mean ± 1.5 standard deviations (SD), indicating the variability of the data around the mean. Outliers are individually marked as points, representing data points that fall outside the range of mean±1.5 SD (see also in Extended Data Fig. 7e and f). Statistical significance was determined using a paired-sample t test. e and f, Boxplots illustrating log₂(pre-piRNA reads per million+1) for WT worms and gei-17 mutants at 25 °C. Statistical significance was determined using a paired-sample t test. g and h, Images displaying GFP::FLAG::Degron::GEI-17 (green) and mCherry::H2B (magenta) in embryos and larval animals.