Extended Data Fig. 1: UAD-2 and USTC form distinct foci corresponding to piRNA Cluster II during spermatogenesis in hermaphrodites and males.
From: piRNA gene density and SUMOylation organize piRNA transcriptional condensate formation
a, Genome-wide distribution of piRNA genes (n = 15365) in C. elegans. b, Diagram illustrating LacI::tagRFP driven by the rps-2 promoter and unc-54 UTR inserted at the LG IV ttti5605 site. c. Diagram illustrating 256x LacO inserted into piRNA cluster II (LG IV position: 15,809,942, contributed by the Christian Frøkjær-Jensen lab). d. Images showing the subcellular localization of UAD-2::GFP and LacI::tagRFP in the nuclei of pachytene germ cells from wild-type animals. All images were taken by the Leica THUNDER imaging System and deconvoluted using Leica Application Suite X software (version 3.7.4.23463). All images are representative of more than three animals (see also in Extended Data Fig. 1e-o). e. Images showing the subcellular localization of TOFU-4::GFP and LacI::tagRFP in the nuclei of pachytene germ cells from wild-type animals. f. Images showing the subcellular localization of UAD-2::GFP and LacI::tagRFP in the nuclei of pachytene germ cells from animals with 256xLacO insertions. g. Images showing the subcellular localization of TOFU-4::GFP and LacI::tagRFP in the nuclei of pachytene germ cells from animals with 256xLacO insertions. h-k. Images displaying the subcellular localization of UAD-2 and USTC components alongside the chromatin marker mCherry::H2B in the spermiogenesis region of the germline in L4 stage hermaphrodite animals. l-o. Images displaying the subcellular localization of UAD-2 and USTC components alongside the chromatin marker mCherry::H2B of the germline in adult male animals.
