Table 3 Primer sequences used for qPCR amplification.
Gene Title | Gene Symbol | primer sequence |
|---|---|---|
Glyceraldehyde-3-phosphate dehydrogenase | GAPDH | F:5¡−-AGAGACAGCCGCATCTTCTTG-3¡− |
R:5¡−-ACCGACCTTCACCATCTTGTCTA-3¡− | ||
Alkaline phosphatase | ALP | F:5¡−-CCGATCGGGACTGGTACTC-3¡− |
R:5¡−-TCAGTTCTGTTCTTGGGGTACA-3¡− | ||
Osteocalcin | OCN | F:5¡−-GAGGGCAGTAAGGTGGTGAA-3¡− |
R:5¡−-GTCCGCTAGCTCGTCACAAT-3¡− | ||
Osteopontin | OPN | F:5¡−-TGTCCTCTGAAGAAACGGATG-3¡− |
R:5¡−-ACAGAATCCTCGCTCTCTGC-3¡− | ||
Bone sialoprotein | BSP | F:5¡−-CGGCCACGCTACTTTCTTTA-3¡− |
R:5¡−-CCCTCCTCCTCCGAACTATC-3¡− | ||
Collagen type I | COLI | F:5¡−-CATGTTCAGCTTTGTGGACCT-3¡− |
R:5¡−-GGTTTCCACGTCTCACCATT-3¡− | ||
Runt-related transcription factor 2 | RunX2 | F:5¡−-CTTCACAAATCCTCCCCAAG-3¡− |
R:5¡−-GAGGCGGTCAGAGAACAAAC-3¡− | ||
Osterix | OSX | F:5¡−-CACTGGCTCCTGGTTCTCTC-3¡− |
R:5¡−-GCTGTTGAGTCTCGCAGAGG-3¡− | ||
Bone morphogenetic protein 2 | BMP2 | F:5¡−-AGACCACCGGCTGGAGAG-3¡− |
R:5¡−-TGAGAAACTCATCAGTAGGGACAG-3¡− | ||
CCAAT/enhancer binding protein, alpha | C/EBPa | F:5¡−-AAGTCGGTGGATAAGAACAGC-3¡− |
R:5¡−-TCAACTCCAACACCTTCTGC-3¡− | ||
Adipocyte lipid-binding protein | AP2 | F:5¡−-GATTTCCTTCAAACTGGGCG-3¡− |
R:5¡−-TGACACATTCCACCACCAGC-3¡− | ||
Lipoprotein lipase | LPL | F:5¡−-CAGCTGGGCCTAACTTTGAG-3¡− |
R:5¡−-AATGGCTTCTCCAATGTTGC-3¡− |
