Figure 1
From: NK cell-derived IL-10 is critical for DC-NK cell dialogue at the maternal-fetal interface
IL-10 rescues pregnancy failure upon NK cell depletion in FLT3-treated dams. (A) IL-10 expression in implantation sites from control, FLT3 treated (FLT3) and FLT3 treated- NK cell depleted (FLT3-dNK) pregnant female mice on ED7.5, as analysed by qRT-PCR. **p < 0.01; as assessed by ANOVA, Tukey multiple comparisons test. (B) The scheme illustrates the experimental design for DC expansion and NK cell depletion during early pregnancy. Upon plug detection; female mice were injected daily with 10 μg FLT3 i.p. + a single anti-asialo GM1 injection on ED4.5 to analyze effects of NK cell depletion upon expansion of DC (FLT3-dNK mice). Recombinant IL-10 (rIL-10) was administrated on ED4.5 and ED5.5. (C) Macroscopical appearance of the uterus and implantations registered during E7.5. The pictures show the normal phenotype of control and FLT3-dNK + rIL-10 implantations in contrast to that observed in FLT3-dNK mice, which exhibit completely resorbed embryos. (D) Microscopical assessment of H&E stained implantation site sections revealed abnormalities in the decidual architecture of dams treated with FLT3 and anti-asialo GM1 (FLT3-dNK), with no distinguishable mesometrial (MD) and antimesometrial pole (AMD) and no signs of developed embryos on ED7.5. Scale: 200 μm. (E) Number of viable and non-viable implantations from dams treated with FTL3 and anti-asialo GM1 (FLT3-dNK) and dams treated also with rIL-10 on ED7.5. Data shown are mean ± SEM derived from six to eight mice per group. **p < 0.001 as analyzed by the Mann-Whitney U test.
